An alkaline follicular fluid fraction induces capacitation and limited release of oviduct epithelium-bound stallion sperm
| Autor: | Leemans, Bart, Gadella, Bart M, Stout, Tom A E, Nelis, Hilde, Hoogewijs, Maarten, Van Soom, Ann, Fertility & Reproduction, Infection & Immunity, dB&C I&I, dES/dFAH FR, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten |
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| Přispěvatelé: | Fertility & Reproduction, Infection & Immunity, dB&C I&I, dES/dFAH FR, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Embryology medicine.medical_specialty endocrine system Time Factors Acrosome reaction Motility Oviducts Biology Andrology Tissue Culture Techniques chemistry.chemical_compound Endocrinology Capacitation Internal medicine medicine Cell Adhesion Animals Horses Phosphorylation Sperm motility urogenital system Acrosome Reaction Obstetrics and Gynecology Tyrosine phosphorylation Epithelial Cells Cell Biology Hydrogen-Ion Concentration Sperm Follicular fluid Spermatozoa Follicular Fluid Calcium Ionophores Reproductive Medicine chemistry Sperm Motility Oviduct Tyrosine Calcium Female Sperm Capacitation |
| Zdroj: | Reproduction, 150(3), 193. BioScientifica Ltd. |
| ISSN: | 1470-1626 |
| Popis: | Induction of hyperactivated motility is considered essential for triggering the release of oviduct-bound mammalian spermatozoa in preparation for fertilization. In this study, oviduct-bound stallion spermatozoa were exposed for 2 h to: i) pre-ovulatory and ii) post-ovulatory oviductal fluid; iii) 100% and iv) 10% follicular fluid (FF); v) cumulus cells, vi) mature equine oocytes, vii) capacitating and viii) non-capacitating medium. None of these triggered sperm release or hyperactivated motility. Interestingly, native FF was detrimental to sperm viability, an effect that was negated by heat inactivation, charcoal treatment and 30 kDa filtration alone or in combination. Moreover, sperm suspensions exposed to treated FF at pH 7.9 but not pH 7.4 showed Ca2+-dependent hypermotility. Fluo-4 AM staining of sperm showed elevated cytoplasmic Ca2+in hyperactivated stallion spermatozoa exposed to treated FF at pH 7.9 compared to a modest response in defined capacitating conditions at pH 7.9 and no response in treated FF at pH 7.4. Moreover, 1 h incubation in alkaline, treated FF induced protein tyrosine phosphorylation in 20% of spermatozoa. None of the conditions tested induced widespread release of sperm pre-bound to oviduct epithelium. However, the hyperactivating conditions did induce release of 70–120 spermatozoa per oviduct explant, of which 48% showed protein tyrosine phosphorylation and all were acrosome-intact, but capable of acrosomal exocytosis in response to calcium ionophore. We conclude that, in the presence of elevated pH and extracellular Ca2+, a heat-resistant, hydrophilic, 2+and hyperactivated motility in stallion sperm, but infrequent release of sperm pre-bound to oviduct epithelium. |
| Databáze: | OpenAIRE |
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