| Popis: |
Changes in the intracellular concentration of free calcium (Ca2+) underpin egg activation and initiation of development in animals and plants. In mammals, the Ca2+release is periodical, known as Ca2+oscillations, and mediated by the type 1 inositol 1,4,5-trisphosphate receptor (IP3R1). Another divalent cation, zinc (Zn2+), increases exponentially during oocyte maturation and is vital for meiotic transitions, arrests, and polyspermy prevention. It is unknown if these pivotal cations interplay during fertilization. Here, using mouse eggs, we showed that basal concentrations of labile Zn2+are indispensable for sperm-initiated Ca2+oscillations because Zn2+-deficient conditions induced by cell-permeable chelators abrogated Ca2+responses evoked by fertilization and other physiological and pharmacological agonists. We also found that chemically- or genetically generated Zn2+-deficient eggs displayed reduced IP3R1 sensitivity and diminished ER Ca2+leak despite the stable content of the stores and IP3R1 mass. Resupplying Zn2+restarted Ca2+oscillations, but excessive Zn2+prevented and terminated them, hindering IP3R1 responsiveness. The findings suggest that a permissive window of Zn2+concentrations is required for Ca2+responses and IP3R1 function in eggs, ensuring optimal response to fertilization and egg activation. |
