'ISA-Lation' of Single-Stranded Positive-Sense RNA Viruses from Non-Infectious Clinical/Animal Samples
| Autor: | Géraldine Piorkowski, Xavier de Lamballerie, Antoine Nougairède, Fabien Aubry, Ernest A. Gould, Lauriane de Fabritus |
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| Přispěvatelé: | Emergence des Pathologies Virales (EPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Emergence des Pathologies Virales ( EPV ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ) -Assistance Publique - Hôpitaux de Marseille ( APHM ), Institut Hospitalier Universitaire Méditerranée Infection ( IHU AMU ), Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ) -Assistance Publique - Hôpitaux de Marseille ( APHM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
DIAGNOSTIC VIROLOGY
INFECTIOUS MOLECULAR CLONES Science viruses Biosecurity lcsh:Medicine Biology medicine.disease_cause [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology Cell Line law.invention CHIKUNGUNYA Mice law Encephalitis Viruses medicine Animals Humans RNA Viruses Chikungunya REAL-TIME PCR lcsh:Science Polymerase chain reaction Subgenomic mRNA Multidisciplinary SEQUENCES lcsh:R RNA AMPLIFICATION Virology SIMIAN IMMUNODEFICIENCY VIRUS 3. Good health GENOME [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine RNA Viral Enterovirus lcsh:Q Sample collection Research Article GENERATION |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (e0138703 ), ⟨10.1371/journal.pone.0138703⟩ PLoS ONE, Public Library of Science, 2015, 10 (e0138703 ), 〈10.1371/journal.pone.0138703〉 PLoS ONE, Vol 10, Iss 9, p e0138703 (2015) Hyper Article en Ligne HAL AMU HAL-IRD DOAJ-Articles INRIA a CCSD electronic archive server Europe PubMed Central PLOS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0138703⟩ |
| Popis: | International audience; Isolation of viral pathogens from clinical and/or animal samples has traditionally relied on either cell cultures or laboratory animal model systems. However, virus viability is notoriously susceptible to adverse conditions that may include inappropriate procedures for sample collection, storage temperature, support media and transportation. Using our recently described ISA method, we have developed a novel procedure to isolate infectious single-stranded positive-sense RNA viruses from clinical or animal samples. This approach, that we have now called "ISA-lation", exploits the capacity of viral cDNA subgenomic fragments to reassemble and produce infectious viral RNA in susceptible cells. Here, it was successfully used to rescue enterovirus, Chikungunya and Tick-borne encephalitis viruses from a variety of inactivated animal and human samples. ISA-lation represents an effective option to rescue infectious virus from clinical and/or animal samples that may have deteriorated during the collection and storage period, but also potentially overcomes logistic and administrative difficulties generated when complying with current health and safety and biosecu-rity guidelines associated with shipment of infectious viral material. |
| Databáze: | OpenAIRE |
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