Effects of enrofloxacin on porcine phagocytic function

Autor: E.J. Schoevers, L.A.M.G. van Leengoed, T. A. Niewold, Jos H. M. Verheijden
Předmět:
Staphylococcus aureus
Pasteurella multocida
Phagocyte
Neutrophils
Swine
animal diseases
Phagocytosis
Antineoplastic Agents
Microbial Sensitivity Tests
Quinolones
Biology
medicine.disease_cause
Microbiology
Minimum inhibitory concentration
Anti-Infective Agents
Macrophages
Alveolar
Enrofloxacin
medicine
Animals
Life Science
Pharmacology (medical)
Mechanisms of Action: Physiological Effects
Instituut voor Dierhouderij en Diergezondheid
Actinobacillus pleuropneumoniae
Antibacterial agent
Pharmacology
Phagocytes
ID-Lelystad
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
ID Lelystad
Infectious Diseases
medicine.anatomical_structure
ID-Lelystad
Instituut voor Dierhouderij en Diergezondheid
ID Lelystad
Institute for Animal Science and Health
Institute for Animal Science and Health
Fluoroquinolones
medicine.drug
Zdroj: Scopus-Elsevier
Antimicrobial Agents and Chemotherapy 43 (1999)
Antimicrobial Agents and Chemotherapy, 43, 2138-2143
ISSN: 0066-4804
Popis: The interaction between enrofloxacin and porcine phagocytes was studied with clinically relevant concentrations of enrofloxacin. Enrofloxacin accumulated in phagocytes, with cellular concentration/extracellular concentration ratios of 9 for polymorphonuclear leukocytes (PMNs) and 5 for alveolar macrophages (AMs). Cells with accumulated enrofloxacin brought into enrofloxacin-free medium released approximately 80% (AMs) to 90% (PMNs) of their enrofloxacin within the first 10 min, after which no further release was seen. Enrofloxacin affected neither the viability of PMNs and AMs nor the chemotaxis of PMNs at concentrations ranging from 0 to 10 μg/ml. Enrofloxacin (0.5 μg/ml) did not alter the capability of PMNs and AMs to phagocytize fluorescent microparticles or Actinobacillus pleuropneumoniae , Pasteurella multocida , and Staphylococcus aureus . Significant differences in intracellular killing were seen with enrofloxacin at 5× the MIC compared with that for controls not treated with enrofloxacin. PMNs killed all S. aureus isolates in 3 h with or without enrofloxacin. Intracellular S. aureus isolates in AMs were less susceptible than extracellular S. aureus isolates to the bactericidal effect of enrofloxacin. P. multocida was not phagocytosed by PMNs. AMs did not kill P. multocida , and similar intra- and extracellular reductions of P. multocida isolates by enrofloxacin were found. Intraphagocytic killing of A. pleuropneumoniae was significantly enhanced by enrofloxacin at 5× the MIC in both PMNs and AMs. AMs are very susceptible to the A. pleuropneumoniae cytotoxin. This suggests that in serologically naive pigs the enhancing effect of enrofloxacin on the bactericidal action of PMNs may have clinical relevance.
Databáze: OpenAIRE
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