ICMSF methods studies. II. Comparison of analytical schemes for detection of Salmonella in high-moisture foods
| Autor: | D. A. Gabis, J. H. Silliker |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Salmonella
Meat Swine Eggs Immunology Thiosulfates Food Contamination Lactose Egg albumen medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Genetics medicine Methods Animals Food science Molecular Biology Chromatography Moisture Chemistry Humidity General Medicine Contamination Culture Media Food Microbiology Cystine Cattle Shigella Chickens |
| Zdroj: | Canadian journal of microbiology. 20(5) |
| ISSN: | 0008-4166 |
| Popis: | Twenty-one naturally contaminated high-moisture food samples were analyzed to compare the relative efficiency of using thirteen 25-g, three 108.3-g, one 325-g, and one 500-g subsamples in recovery of salmonellae. In addition, recovery by preenrichment in lactose broth was compared with direct selective enrichment in selenite cystine and tetrathionate brilliant green broths. Analysis of preenriched large lumped subsamples, prepared by combining multiple 25-g subsamples, detected positive samples as effectively as the analysis of individual 25-g subsamples. In the analysis of 25-g subsamples, direct enrichment detected as many positive "lots" as preenrichment, but in the analysis of lumped subsamples, direct enrichment gave dramatically reduced recovery. Nevertheless, directly enriched 25-g subsamples resulted in a significantly lower rate of recovery than preenriched subsamples. The results indicate that analysis of a single lumped 325-g preenriched subsample is as efficient in the recovery of salmonellae as the analysis of thirteen 25-g preenriched subsamples. Further, it is apparent that preenrichment of high-moisture foods, such as meat, poultry, and eggs is to be recommended over direct enrichment for the detection of salmonellae. |
| Databáze: | OpenAIRE |
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