Alterations in Blood Monocyte Functions in Parkinson's Disease

Autor: Holger Jon Møller, Claudia Schulte, Daniela Berg, Daniel E. Otzen, Kalpana Shrivastava, Sara K. Nissen, Walter Maetzler, David Goldeck, Marina Romero-Ramos
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
metabolism [Antigens
CD]
pathology [Leukocytes
Mononuclear]
metabolism [Receptors
Cell Surface]
Parkinson's disease
alpha-synuclein
medicine.medical_treatment
Cell Count
metabolism [Microglia]
0302 clinical medicine
cytokine
Medicine
metabolism [alpha-Synuclein]
Sex Characteristics
education.field_of_study
Microglia
Parkinson Disease
medicine.anatomical_structure
Cytokine
Neurology
monocyte
alpha-Synuclein
Cytokines
Female
CD14
Population
metabolism [Parkinson Disease]
Antigens
Differentiation
Myelomonocytic
Receptors
Cell Surface
metabolism [Leukocytes
Mononuclear]
Peripheral blood mononuclear cell
03 medical and health sciences
Immune system
Antigens
CD
Humans
ddc:610
education
business.industry
Monocyte
metabolism [Cytokines]
metabolism [Antigens
Differentiation
Myelomonocytic]
pathology [Parkinson Disease]
030104 developmental biology
Immunology
Leukocytes
Mononuclear
CD163
Neurology (clinical)
business
030217 neurology & neurosurgery
Zdroj: Nissen, S K, Shrivastava, K, Schulte, C, Otzen, D E, Goldeck, D, Berg, D, Møller, H J, Maetzler, W & Romero-Ramos, M 2019, ' Alterations in Blood Monocyte Functions in Parkinson's Disease ', Movement Disorders, vol. 34, no. 11, pp. 1711-1721 . https://doi.org/10.1002/mds.27815
Movement disorders 34(11), 1711-1721 (2019). doi:10.1002/mds.27815
ISSN: 1531-8257
0885-3185
Popis: BACKGROUND: PD is a multisystem disease where both central and peripheral nervous systems are affected. This systemic involvement also includes the immune response in PD, which implicates not only microglia in the brain, but also peripheral immune cells, such as monocytes; however, this aspect has been understudied.OBJECTIVES: The purpose of this study was to investigate the PD-related changes in peripheral immune cells, their responsiveness to stimulation, and their ability to release immunomodulatory molecules that might have consequences for the disease progression.METHODS: Using flow cytometry, we investigated the monocytic population in peripheral blood mononuclear cells from PD patients and healthy individuals. We also evaluated the in vitro response to inflammogen lipopolysaccharides and to fibrillar α-synuclein by measuring the expression of CD14, CD163, and HLA-DR and by analysis of soluble immune-related molecules in the supernatant.RESULTS: Peripheral blood immune cells from PD patients had lower survival in culture, but showed a higher monocytic proliferative ability than control cells, which was correlated with shorter disease duration and late disease onset. In addition, PD patients' cells were less responsive to stimulation, as shown by the lack of changes in CD163 and CD14 expression, and by the absence of significant upregulation of anti-inflammatory cytokines in culture. Moreover, PD peripheral immune cells shed lower in vitro levels of soluble CD163, which suggests a less responsive monocytic population and/or an activation status different from control cells. Interestingly, some of the results were sex associated, supporting a differential immune response in females versus males.CONCLUSIONS: Our data suggest that PD involves monocytic changes in blood. These cells show reduced viability and are unresponsive to specific stimuli, which might have a relevant consequence for disease progression. © 2019 International Parkinson and Movement Disorder Society.
Databáze: OpenAIRE
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