Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components against Oxidative Stress In Vitro

Autor: Monika A. Olszewska, Pawel Nowak, Agnieszka Kicel, Malgorzata Sopinska, Pawel Ciszewski, Anna Marchelak, Aleksandra Owczarek, Joanna Kolodziejczyk-Czepas
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Antioxidant
DPPH
medicine.medical_treatment
Pharmaceutical Science
medicine.disease_cause
Analytical Chemistry
Lipoxygenase
chemistry.chemical_compound
Plasma
Drug Discovery
oxidative stress
Enzyme Inhibitors
Cotoneaster
biology
Chemistry
Biological activity
Lipoxygenases
Blood proteins
antioxidants
Proanthocyanidin
Biochemistry
Chemistry (miscellaneous)
Molecular Medicine
Inflammation Mediators
Hyaluronoglucosaminidase
hyaluronidase
In Vitro Techniques
Protective Agents
Article
peroxynitrite
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
medicine
Humans
Physical and Theoretical Chemistry
Rosaceae
Inflammation
human plasma
Plant Extracts
Organic Chemistry
Polyphenols
lipoxygenase
LC-MS
Plant Leaves
030104 developmental biology
Polyphenol
biology.protein
Oxidative stress
Zdroj: Molecules, Vol 23, Iss 10, p 2472 (2018)
Molecules
Volume 23
Issue 10
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
ISSN: 1420-3049
Popis: The present study investigated the phenolic profile and biological activity of dry extracts from leaves of C. bullatus, C. zabelii and C. integerrimus&mdash
traditional medicinal and dietary plants&mdash
and evaluated their potential in adjunctive therapy of cardiovascular diseases. Complementary UHPLC-PDA-ESI-MS3, HPLC-PDA-fingerprint, Folin-Ciocalteu, and n-butanol/HCl assays of the extracts derived by fractionated extraction confirmed that they are rich in structurally diverse polyphenols (47 analytes, content up to 650.8 mg GAE/g dw) with proanthocyanidins (83.3&ndash
358.2 mg CYE/g) dominating in C. bullatus and C. zabelii, and flavonoids (53.4&ndash
147.8 mg/g) in C. integerrimus. In chemical in vitro tests of pro-inflammatory enzymes (lipoxygenase, hyaluronidase) inhibition and antioxidant activity (DPPH, FRAP), the extracts effects were dose-, phenolic- and extraction solvent-dependent. The most promising polyphenolic extracts were demonstrated to be effective antioxidants in a biological model of human blood plasma&mdash
at in vivo-relevant levels (1&ndash
5 µ
g/mL) they normalized/enhanced the non-enzymatic antioxidant capacity of plasma and effectively prevented peroxynitrite-induced oxidative/nitrative damage of plasma proteins and lipids. As demonstrated in cytotoxicity tests, the extracts were safe&mdash
they did not affect viability of human peripheral blood mononuclear cells. In conclusion, Cotoneaster leaves may be useful in development of natural-based products, supporting the treatment of oxidative stress/inflammation-related chronic diseases, including cardiovascular disorders.
Databáze: OpenAIRE
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