Polymeric gene carriers bearing pendant β-cyclodextrin: the relevance of glycoside permethylation on the 'in vitro' cell response
| Autor: | Alberto Gallardo, Rodrigo Navarro, Mónica Pérez-Perrino, Alfonso Fernández-Mayoralas, Abhay Pandit, José Luis López-Lacomba, Laetitia Plet, Enrique Martínez-Campos, Carlos Elvira, Guillermo Corrales, Juan Alfonso Redondo, Helmut Reinecke |
|---|---|
| Přispěvatelé: | European Commission, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2016 |
| Předmět: |
nonviral gene vectors
Polymers and Plastics Cationic polymers Polymers pyrrolidine Library science Biocompatible Materials click clusters 02 engineering and technology European Social Fund 010402 general chemistry delivery vectors Methylation 01 natural sciences Mice Gene therapy Cations Political science Materials Chemistry Gamma-cyclodextrin Animals European commission Glycosides alpha-cyclodextrin Acrylamides Cyclodextrins therapy cyclodextrins Gene carrier beta-Cyclodextrins Organic Chemistry cholesterol 3T3 Cells 021001 nanoscience & nanotechnology polyamidoamine dendrimer gene therapy 0104 chemical sciences Microscopy Fluorescence transfection Research council Spectrophotometry Ultraviolet Cell response 0210 nano-technology gamma-cyclodextrins Nonviral gene vectors cationic polymers Plasmids |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname BASE-Bielefeld Academic Search Engine |
| Popis: | The incorporation of cyclodextrins (CDs) to nonviral cationic polymer vectors is very attractive due to recent studies that report a clear improvement of their cytocompatibility and transfection efficiency. However, a systematic study on the influence of the CD derivatization is still lacking. In this work, the relevance of β-CD permethylation has been addressed by preparing and evaluating two series of copolymers of the cationic N-ethyl pyrrolidine methacrylamide (EPA) and styrenic units bearing pendant hydroxylated and permethylated β-CDs (HCDSt and MeCDSt, respectively). For both cell lines, CDs permethylation shows a strong influence on plasmid DNA complexation, >in vitro> cytocompatibility and transfection efficiency of the resulting copolymers over two murine cell lines. While the incorporation of the hydroxylated CD moiety increased the cytotoxicity of the copolymers in comparison with their homopolycationic counterpart, the permethylated copolymers have shown full cytocompatibility as well as superior transfection efficiency than the controls. This behavior has been related to the different chemical nature of both units and tentatively to a different distribution of units along the polymeric chains. Cellular internalization analysis with fluorescent copolymers supports this behavior. The authors gratefully acknowledge support from Grants MAT-2010-20001, MAT2013-42957-R, CSD2009-00088 (Consolider project), SAF2011-27863, 201280E091 (CSIC intramural project) and PI10/02985FIS (Seventh Framework Programme of the European Commission, EuroNanomed ERA-NET initiative, REBONE project). J.A.R. and R.N. gratefully acknowledges funding from the Spanish Research Council (CSIC) and European Social Fund (ESF) through the JAE program |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|