Adeno-associated virus transformation into the normal miniature pig and the normal guinea pigs cochlea via scala tympani
| Autor: | Weiwei Guo, Yue Zhang, Xunbei Shi, Shiming Yang, Nan Wu, Chang Lin |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Miniature pig Spiral limbus Swine Guinea Pigs Biology Transfection Green fluorescent protein Andrology 03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases medicine Animals Hearing Loss Cochlea Round window Hair Cells Auditory Inner General Medicine Anatomy Genetic Therapy Dependovirus Perilymph biology.organism_classification 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Spiral ligament Swine Miniature sense organs 030217 neurology & neurosurgery |
| Zdroj: | Acta oto-laryngologica. 137(9) |
| ISSN: | 1651-2251 |
| Popis: | To investigate the expression of the miniature pig cochlea after AAV1 transfect into the cochlea via round window membrane (RWM).Twenty miniature pigs are equally divided into four experimental groups. Twelve miniature pigs are equally divided into four control groups. Each pig was transfected with the AAV1 in the experimental group via RWM and each pig was transduced with the artificial perilymph in the control group. The expression of green fluorescent protein (GFP) was observed at 2 weeks, 3 weeks and 4 weeks, respectively. Likewise, AAV1 was delivered into the guinea pigs cochleas using the same method, and the results were compared with that of the miniature pigs.The expression was mainly in the inner hair cells of the miniature pig. The expression of GFP began to appear at 2 weeks, reached the peak at 3 weeks. It also expressed in Hensen's cells, inner pillar cells, outer pillar cells, spiral limbus, and spiral ligament. In the meanwhile, AAV1 was delivered into guinea pig cochlea via the same method, and AAV1 was also expressed in the inner hair cells. But the expression peaked at 2 weeks, and the efficiency of the inner hair cell transfection was higher than that of the pig.AAV1 can be transformed into miniature pig cochlea via scala tympani by the RWM method efficiently. |
| Databáze: | OpenAIRE |
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