Orthogonal Engineering of Biosynthetic Pathway for Efficient Production of Limonene in Saccharomyces cerevisiae
| Autor: | Ying-Jin Yuan, Dengwei Lei, Guozhen Jiang, Guang-Rong Zhao, Jin-Lai Zhang, Xue Liu, Jianjun Qiao, Jihua Wu, Si Cheng |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Citrus Saccharomyces cerevisiae Proteins Monoterpene Saccharomyces cerevisiae Glucose Transport Proteins Facilitative Biomedical Engineering Heterologous 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Biomass Intramolecular Lyases Promoter Regions Genetic Gene Plant Proteins 030304 developmental biology 0303 health sciences Limonene biology Chemistry Substrate (chemistry) Geranyltranstransferase General Medicine biology.organism_classification Terpenoid Metabolic Engineering Biochemistry Batch Cell Culture Techniques Fermentation Plasmids |
| Zdroj: | ACS Synthetic Biology. 8:968-975 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.9b00135 |
| Popis: | Limonene, a plant-derived natural cyclic monoterpene, is widely used in the pharmaceutical, food, and cosmetics industries. The conventional limonene biosynthetic (CLB) pathway in engineered Saccharomyces cerevisiae consists of heterologous limonene synthase (LS) using endogenous substrate geranyl diphosphate (GPP) and suffers from poor production of limonene. In this study, we report on an orthogonal engineering strategy in S. cerevisiae for improving the production of limonene. We reconstructed the orthogonal limonene biosynthetic (OLB) pathway composed of SlNDPS1 that catalyzes IPP and DMAPP to NPP ( cis-GPP) and plant LS that converts NPP to limonene. We find that the OLB pathway is more efficient for production of limonene than the CLB pathway. When expression of the competing gene ERG20 was chromosomally regulated by the glucose-sensing promoter HXT1, the OLB pathway-harboring strain produced 917.7 mg/L of limonene in fed-batch fermentation, a 6-fold increase of the CLB pathway, representing the highest titer reported to date. Orthogonal engineering exhibits great potential for production of terpenoids in S. cerevisiae. |
| Databáze: | OpenAIRE |
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