Identification of genes expressed in retinal progenitor/stem cell colonies isolated from the ocular ciliary body of adult mice
| Autor: | Julie Lord-Grignon, Mohamed Abdouh, Gilbert Bernier |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
PAX6 Transcription Factor
Mice Transgenic Nerve Tissue Proteins In situ hybridization Biology Retina chemistry.chemical_compound Mice Ciliary body Genetics medicine Animals Paired Box Transcription Factors Eye Proteins Molecular Biology Homeodomain Proteins Microphthalmia-Associated Transcription Factor Eye morphogenesis cDNA library Stem Cells Ciliary Body HMGA2 Protein Gene Expression Regulation Developmental Membrane Proteins Retinal Embryo Mammalian Molecular biology Embryonic stem cell Mice Inbred C57BL Repressor Proteins medicine.anatomical_structure chemistry Trans-Activators PAX6 Stem cell Developmental Biology |
| Zdroj: | Gene expression patterns : GEP. 6(8) |
| ISSN: | 1567-133X |
| Popis: | Rare pigmented cells showing retinal stem cell characteristics have been identified in the ocular ciliary body (CB) of adult mammals. In vitro, these cells were reported to clonally proliferate and generate pigmented sphere colonies (PSC) containing multipotent retinal progenitor-like cells. Because these cells may have important clinical applications and because their embryonic origin is unclear, we have analyzed their local environment and gene expression profile. We found that transcription factors Pax6, Six3, and Rx, all involved in early eye morphogenesis, were expressed in the CB of adult mice. By sequencing a PSC cDNA library, we found that PSC expressed at high levels transcripts involved in the control of redox metabolism and cellular proliferation. PSC also expressed the retinal transcription factor Six6, which expression was not detected in the CB epithelium. By in situ hybridization screen, we found that Palmdelphin (Palm), Hmga2, and a novel transcript were expressed in the central nervous system of early embryos. Palm expression delineated the pigmented epithelium of the future CB and the developing myotome. Hmga2 was expressed in the ventricular zone of the telencephalon, the developing retinal ciliary margin and lens. Several genes expressed in PSC were also expressed in the nasal anlagen. Taken together, our study reveals that PSC isolated from the ocular CB express genes involved in the control of embryonic development, retinal identity, redox metabolism, and cellular proliferation. |
| Databáze: | OpenAIRE |
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