Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses
| Autor: | John K. Nyanjui, Roger Pelle, Yoshikazu Honda, Guy H. Palmer, Jocelyn Bray, James Gachanja, Waithaka Mwangi, Duncan Mwangi, Wendy C. Brown, Evans L. N. Taracha, Simon P. Graham |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Protozoan Vaccines T cell Theileria parva Immunology Antigens Protozoan chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Biology Lymphocyte Activation DNA vaccination Interferon-gamma Immune system Adjuvants Immunologic Antigen Vaccines DNA medicine Animals Cytotoxic T cell General Veterinary Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins biology.organism_classification Virology Recombinant Proteins Theileriasis CTL medicine.anatomical_structure Cattle CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Veterinary Immunology and Immunopathology. 140:244-251 |
| ISSN: | 0165-2427 |
| Popis: | Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4(+) and CD8(+) T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect