Evolutionary interplay of single nucleotide polymorphisms at the promoter region of TNF-α gene in different clinical outcomes of malaria in India
| Autor: | Stuti Mohanty, Aparup Das, Veena Pande, Akshaya K Mohanty, Upasana Shyamsunder Singh, Sanjib Mohanty |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Genetic Linkage 030106 microbiology Malaria Cerebral India Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Microbiology Linkage Disequilibrium Evolution Molecular 03 medical and health sciences parasitic diseases Genetics medicine Humans SNP Genetic Predisposition to Disease Allele Promoter Regions Genetic Molecular Biology Gene Alleles Ecology Evolution Behavior and Systematics Tumor Necrosis Factor-alpha Heterozygote advantage Promoter medicine.disease Malaria Patient Outcome Assessment 030104 developmental biology Infectious Diseases Haplotypes Cerebral Malaria Female Morbidity |
| Zdroj: | Infection, Genetics and Evolution. 69:107-116 |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2019.01.025 |
| Popis: | Host genetic factors are frequently ascribed to differential malaria outcomes as a by-product of evolutionary adaptation. To this respect, Tumor Necrosis factor alpha (TNF-α), a human cytokine, is known to be associated with malaria through its differential regulation in diverse malaria manifestations. Since diversity in differential malaria outcome is uncommon in every endemic settings, possible association of TNF-α and malaria is not commonly established. In order to check for association between the occurrence of Single Nucleotide Polymorphisms (SNPs) in the TNF-α gene with different malaria manifestations, we have sequenced a 4011 bp region constituting the promoter and the whole gene of human TNF-α in 61 patients [(16 cerebral plus severe (SCM), 21 severe (SM) and 24 uncomplicated (UM)] samples in a highly malaria endemic state (Odisha) of India. Multiple sequence alignment revealed presence of six SNPs (−1031 T > C, −863C > A, −857C > T, −308G > A, −806C > T, +787C > A), out of which the -806C > T and +787C > A are novel in malaria patients in general and the +787C > A was detected for the first time in humans. Although alleles due to six different SNPs segregate differentially in the three groups of malaria (SCM, SM and UM) in the present study, interestingly, for the −1031 T > C position, the frequency of individuals possessing the homozygous rare allele was higher in the SCM group with a higher number of heterozygotes in the UM group. The Tajima's D values considering all the SNPs in a defined group were positive and statistically insignificant conforming no evolutionary constraint. However, statistically significant deviation from expectation under Hardy-Weinberg equilibrium for -1031 T > C SNP in the UM group points towards the probable role of natural selection providing some kind of protection to malaria in Odisha, India. |
| Databáze: | OpenAIRE |
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