Metabolism-associated genome-wide epigenetic changes in bovine oocytes during early lactation
| Autor: | Christiane Neuhoff, Mikhael Poirier, Dessie Salilew-Wondim, Samuel Gebremedhn, Michael Hoelker, Karl Schellander, Tsige Hailay, Franca Rings, Dawit Tesfaye |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Epigenetic memory Bisulfite sequencing lcsh:Medicine Biology Article Epigenesis Genetic Andrology 03 medical and health sciences chemistry.chemical_compound Lactation Epigenetic Profile medicine Animals Epigenetics lcsh:Science 2. Zero hunger Genome Multidisciplinary Fatty acid metabolism lcsh:R Postpartum Period 0402 animal and dairy science 04 agricultural and veterinary sciences DNA Methylation 040201 dairy & animal science 030104 developmental biology medicine.anatomical_structure Differentially methylated regions Risk factors Gene Expression Regulation chemistry Metabolome Oocytes lcsh:Q Cattle CpG Islands Female Genomic imprinting Postpartum period |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Dietary intake in early lactating cows is outmatched by milk production. These cows experience a negative energy balance, resulting in a distinct blood metabolism and poor reproductive function due to impaired ovulation and increased embryo loss. We hypothesize that oocytes from lactating cows undergoing transient metabolic stress exhibit a different epigenetic profile crucial for developmental competence. To investigate this, we collected oocytes from metabolically-profiled cows at early- and mid-postpartum stages and characterized their epigenetic landscape compared with control heifers using whole-genome bisulfite sequencing. Early-postpartum cows were metabolically deficient with a significantly lower energy balance and significantly higher concentrations of non-esterified fatty acids and beta-hydroxybutyrate than mid-postpartum animals and control heifers. Accordingly, 32,990 early-postpartum-specific differentially methylated regions (DMRs) were found in genes involved in metabolic pathways, carbon metabolism, and fatty acid metabolism, likely descriptive of the epigenetic regulation of metabolism in early-postpartum oocytes. DMRs found overlapping CpG islands and exons of imprinted genes such as MEST and GNAS in early-postpartum oocytes suggest that early lactation metabolic stress may affect imprint acquisition, which could explain the embryo loss. This whole-genome approach introduces potential candidate genes governing the link between metabolic stress and the reproductive outcome of oocytes. |
| Databáze: | OpenAIRE |
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