Stimulation of rat liver lysosomal lipase by lipid and by acidic phospholipids
| Autor: | Arnold Kaplan, Masao Kariya |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Male
Hot Temperature government.form_of_government Biophysics Stimulation Endogeny Mitochondria Liver Phosphatidylinositols Biochemistry chemistry.chemical_compound Surface-Active Agents Drug Stability Animals Lipase Molecular Biology Phospholipids Triglycerides Differential centrifugation Triglyceride lipase Lipoprotein lipase Chromatography biology Chemistry Phosphatidylethanolamines Brain Lysophosphatidylcholines Cell Biology Phosphatidic acid Lipids Rats Enzyme Activation Liver biology.protein Lysosomal Lipase government Microsomes Liver Phosphatidylcholines lipids (amino acids peptides and proteins) Lysosomes Subcellular Fractions |
| Zdroj: | Biochemical and biophysical research communications. 44(5) |
| ISSN: | 0006-291X |
| Popis: | Summary Triglyceride lipase of lysosomes purified from rat liver is stimulated by endogenous lipid. Preparation of the lipase activity, in soluble form free of factor, has been accomplished by a two step differential centrifugation. The particle bound, lipase stimulating factor of rat liver homogenates is heat stable (10 min at 100°) and non-dialyzable. It can be solubilized in 2% Triton X100 by heating at 70° for 10 min, and is extractable by and recoverable from lipid solvents. The soluble lipase is stimulated 6-fold by phosphatidic acid and 20-fold by brain fractions containing either phosphatidyl inositide or phosphatidyl serine. |
| Databáze: | OpenAIRE |
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