Alphavirus replicon particles encoding the fusion or attachment glycoproteins of respiratory syncytial virus elicit protective immune responses in BALB/c mice and functional serum antibodies in rhesus macaques
| Autor: | John H. Eldridge, Michael A. Egan, Nicole B. Terio, Krista J. Melville, Deborah Long, Amara Luckay, Lee Anne C. Boutilier, Christopher L. Parks, Rashed Abdullah, Stephen A. Udem, Robert A. Lerch, Matthew B. Elliott, Tong Chen, Siew-Yen Chong, Gerald E. Hancock |
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| Rok vydání: | 2007 |
| Předmět: |
Male
viruses T cell T-Lymphocytes Alphavirus Respiratory Syncytial Virus Infections Biology medicine.disease_cause Antibodies Viral Injections Intramuscular Virus BALB/c Encephalitis Virus Venezuelan Equine Mice Immune system Neutralization Tests medicine Respiratory Syncytial Virus Vaccines Animals Replicon Lung Viral Structural Proteins Mice Inbred BALB C Vaccines Synthetic General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health virus diseases Viral Vaccines respiratory system biology.organism_classification Virology Macaca mulatta Respiratory Syncytial Viruses Infectious Diseases medicine.anatomical_structure Venezuelan equine encephalitis virus biology.protein Molecular Medicine Cytokines Female Antibody Spleen |
| Zdroj: | Vaccine. 25(41) |
| ISSN: | 0264-410X |
| Popis: | Respiratory syncytial virus (RSV) is a major cause of acute respiratory tract disease in humans. Towards development of a prophylactic vaccine, we genetically engineered Venezuelan equine encephalitis virus (VEEV) replicons encoding the fusion (Fa) or attachment (Ga or Gb) proteins of the A or B subgroups of RSV. Intramuscular immunization with a formulation composed of equal amounts of each replicon particle (3vRSV replicon vaccine) generated serum neutralizing antibodies against A and B strains of RSV in BALB/c mice and rhesus macaques. When contrasted with purified natural protein or formalin-inactivated RSV formulated with alum, the 3vRSV replicon vaccine induced balanced Th1/Th2 T cell responses in mice. This was evident in the increased number of RSV-specific IFN-gamma(+) splenocytes following F or G peptide stimulation, diminished quantity of eosinophils and type 2 T cell cytokines in the lungs after challenge, and increased in vivo lysis of RSV peptide-loaded target cells. The immune responses in mice were also protective against intranasal challenge with RSV. Thus, the replicon-based platform represents a promising new strategy for vaccines against RSV. |
| Databáze: | OpenAIRE |
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