Ethanol toxicity differs depending on the time of day
| Autor: | Carolina Bello, Juan Fernando Paredes, Greta Carmona-Antoñanzas, Luisa Vera, Francisco Javier Sánchez-Vázquez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Life Cycles Physiology Gene Expression lcsh:Medicine Toxicology Pathology and Laboratory Medicine chemistry.chemical_compound 0302 clinical medicine Larvae Drug Metabolism Medicine and Health Sciences lcsh:Science Zebrafish Morning Multidisciplinary Behavior Animal Organic Compounds Eukaryota Animal Models Circadian Rhythm Enzymes Chemistry Experimental Organism Systems Osteichthyes Toxicity Physical Sciences Vertebrates Detoxification Research Article Death Rates Biology Research and Analysis Methods 03 medical and health sciences Model Organisms Population Metrics Zeitgeber Genetics Animals Pharmacokinetics Circadian rhythm Adverse effect Swimming Pharmacology Ethanol Population Biology Organic Chemistry lcsh:R Chemical Compounds Organisms Biology and Life Sciences biology.organism_classification 030104 developmental biology Fish chemistry Alcohols lcsh:Q 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE, Vol 13, Iss 1, p e0190406 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Ethanol is one of the most commonly abused drugs and consequently its toxic and psychoactive effect has been widely investigated, although little is known about the time-dependent effects of this drug. In the present research zebrafish was used to assess daily rhythms in ethanol toxicity and behavioural effects, as well as the temporal pattern of expression of key genes involved in ethanol detoxification in the liver (adh8a, adh5, aldh2.1 and aldh2.2). Our results showed marked differences in the mortality rate of zebrafish larvae depending on the time of day of the exposure to 5% ethanol for 1h (82% and 6% mortality in the morning and at night, respectively). A significant daily rhythm was detected with the acrophase located at "zeitgeber" time (ZT) = 04:22 h. Behavioural tests exposing zebrafish to 1% ethanol provoked a major decrease in swimming activity (68-84.2% reduction) at ZT2, ZT6 and ZT10. In contrast, exposure at ZT18 stimulated swimming activity (27% increase). During the day fish moved towards the bottom of the tank during ethanol exposure, whereas at night zebrafish increased their activity levels right after the exposure to ethanol. Genes involved in ethanol detoxification failed to show significant daily rhythms in LD, although all of them exhibited circadian regulation in constant darkness (DD) with acrophases in phase and located at the end of the subjective night. Taken altogether, this research revealed the importance of considering the time of day when designing and carrying out toxicological and behavioural tests to investigate the effects of ethanol, as the adverse effects of this drug were more marked when fish were exposed in the morning than at night. |
| Databáze: | OpenAIRE |
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