RepoMan stimulates the chromosome-dependent pathway of microtubule assembly
| Autor: | Mathieu Bollen, Gerd Van der Hoeven, Sofie De Munter |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Scaffold protein Cell Cycle Proteins Importin Biology Transfection Microtubules 03 medical and health sciences spindle assembly 0302 clinical medicine Microtubule Cyclin-dependent kinase Protein Phosphatase 1 NuSAP1 Chromosomes Human Humans Phosphorylation Enhancer Molecular Biology Mitosis Centrosome TPX2 Nuclear Proteins Cell Biology PP1 Cell biology 030104 developmental biology HEK293 Cells NuMA 030220 oncology & carcinogenesis biology.protein RepoMan M Phase Cell Cycle Checkpoints Carrier Proteins Microtubule-Associated Proteins Developmental Biology HeLa Cells Signal Transduction Research Paper |
| Zdroj: | Cell Cycle |
| ISSN: | 1551-4005 |
| Popis: | RepoMan is a chromosome-associated scaffold protein that integrates signaling of multiple kinases and phosphatases to coordinate spindle-kinetochore interactions, chromosome (de)condensation and nuclear envelope (dis)assembly during mitosis. Another key mitotic event is the assembly of a microtubule-based spindle, which involves redundant pathways emanating from the centrosomes, microtubules and chromosomes. Here we describe a novel mitotic function of RepoMan in regulating chromosome-dependent microtubule assembly. At limiting concentrations of microtubule-destabilizing agents, RepoMan-depleted cells showed enhanced chromosome clustering. This clustering was completely dependent on the partial inhibition of microtubule growth originating from the chromosome-dependent pathway. We also demonstrated that RepoMan interacts with prime regulators of the chromosome-dependent spindle assembly such as NuSAP1, NuMA, and TPX2. In addition, RepoMan was required to enable or maintain phosphorylation of NuSAP1 at CDK sites, thereby enabling activation of NuSAP1 through dissociation of inhibitory importin β/7. Our data identify RepoMan as an enhancer of microtubule assembly at chromosomes. ispartof: CELL CYCLE vol:19 issue:22 pages:3029-3041 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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