Proliferation of T-cell subsets that contact tumour cells in colorectal cancer
| Autor: | S J C Golby, C Chinyama, Jo Spencer |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Stromal cell Adolescent T cell Immunology Cell Communication Lymphocyte Activation Interleukin 21 Lymphocytes Tumor-Infiltrating Antigen Antigens Neoplasm T-Lymphocyte Subsets Clinical Studies medicine Humans Immunology and Allergy Cytotoxic T cell Child Aged Aged 80 and over CD40 biology Middle Aged medicine.anatomical_structure biology.protein Intraepithelial lymphocyte Female Stromal Cells Colorectal Neoplasms CD8 |
| Zdroj: | Clinical and Experimental Immunology. 127:85-91 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2002.01730.x |
| Popis: | SummaryWe have investigated the proliferation rates of T-cell subsets in colorectal carcinomas using immunohistochemistry. It was found that the tumour-infiltrating T cells in contact with the tumour cells have a significantly higher frequency of proliferation than those in the stroma. In particular, the CD8+ intraepithelial lymphocytes (T-IEL) within the tumours have a significantly higher frequency of proliferation in comparison with CD8+ T cells in the stromal compartment or in any normal mucosal lymphoid tissues. It is possible that the proliferation of the CD8+ T-IEL may be driven by self-antigens expressed on the tumour cells. The proportion of CD3+ CD7– T cells is increased within carcinomas compared with the normal colon, and a population of CD57+ T cells was observed which is absent from the normal colon. It is possible that these phenotypes are acquired in situ due to repeated stimulation of the T cells by tumour antigens. Intact colorectal carcinoma explants were cultured, and the presence of tumour-infiltrating T cells analysed after 3 days of culture in isolation from the systemic compartments. CD3+ T cells were proliferating (at a low rate) within the explants after 3 days of culture, indicating that they may be sustained by factors present in the tumour microenvironment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|