Occurrence and seasonal variation of equine estrogens, equilin and equilenin, in the river water of Japan: Implication with endocrine-disrupting potentials to Japanese medaka (Oryzias latipes)
| Autor: | Keisuke Yoshimoto, Masaya Uchida, Yuji Takao, Yasuhiro Ishibashi, Ryoko Yamamoto, Yuta Imamura, Koji Arizono, Masahito Kawai, Nobuaki Tominaga, Nobuhiro Ichikawa, Hiroshi Ishibashi, Yoshinori Ikenaka |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Receptors Steroid Climate Health Toxicology and Mutagenesis Oryzias Gene Expression Estrogen receptor Fresh Water Endocrine Disruptors 010501 environmental sciences Toxicology 01 natural sciences Vitellogenins Japan media_common Reproduction Pregnane X Receptor General Medicine Japanese Medaka Pollution Liver Female Seasons Environmental Monitoring medicine.drug media_common.quotation_subject Endocrine System Biology Andrology 03 medical and health sciences Vitellogenin Rivers medicine Animals Horses Equilin 0105 earth and related environmental sciences Equilenin Hatching Estrogen Receptor alpha Estrogens biology.organism_classification Fertility 030104 developmental biology biology.protein Water Pollutants Chemical |
| Zdroj: | Environmental Pollution. 239:281-288 |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2018.04.029 |
| Popis: | In this study, we determined the concentration of equine estrogens, such as equilin (Eq) and equilenin (Eqn), in the river water collected from nine research stations in Hokkaido, Japan. The LC–MS/MS analysis revealed that Eq concentrations were 2.7 ± 6.7, 0.22 ± 0.12, and 1.2 ± 0.64 ng/L in Sep 2015, Feb 2016, and Jul 2016, respectively. Eqn had concentration levels similar to those of Eq. Comparison of the concentrations at nine research stations showed that seasonal variation was observed in the detected Eq and Eqn concentration levels. This study was the first to show the occurrences and seasonal variation of Eq and Eqn in the river water of Japan. We further investigated the reproductive and transgenerational effects of Eq in Japanese medaka (Oryzias latipes) exposed to 10, 100, and 1000 ng/L for 21 days and assessed the transcriptional profiles of the estrogen-responsive genes in the livers of both sexes. The reproduction assay demonstrated that 1000 ng/L of Eq adversely affected the reproduction (i.e. fecundity) in the F0 generation and that the hatching of F1 generation fertilized eggs was reduced in the 100 and 1000 ng/L treatment groups. Our qRT-PCR assay revealed that the mRNA expression levels of hepatic vitellogenin 1 and 2, choriogenin L and H, and estrogen receptor α were significantly up-regulated in males exposed to 100 and/or 1000 ng/L of Eq. In contrast, the transcriptional levels of several genes, such as pregnane X receptor and cytochrome P450 3A, were down-regulated in the livers of males after the 21-d exposure. These results suggest that Eq has endocrine-disrupting potential such as reproductive and transgenerational effects by the modulation of hepatic estrogen-responsive genes expression on medaka. |
| Databáze: | OpenAIRE |
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