Oligonucleotide transport in rat and human intestine Ussing chamber models
| Autor: | William H. Barr, Susanna Wu-Pong, Barry Dvorchik, Virginia Livesay |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Colon Oligonucleotides Biological Transport Active Pharmaceutical Science Ileum In Vitro Techniques Biology Permeability Intestinal absorption chemistry.chemical_compound medicine Fluorescence microscope Animals Humans Mannitol Pharmacology (medical) ATP Binding Cassette Transporter Subfamily B Member 1 Intestinal Mucosa Rats Wistar Transcellular Fluorescein isothiocyanate Pharmacology Microscopy Confocal Tight junction Ussing chamber General Medicine Rats Jejunum medicine.anatomical_structure Intestinal Absorption chemistry Biochemistry Paracellular transport Biophysics Algorithms |
| Zdroj: | Biopharmaceutics & Drug Disposition. 20:411-416 |
| ISSN: | 1099-081X 0142-2782 |
| DOI: | 10.1002/1099-081x(199912)20:9<411::aid-bdd208>3.0.co;2-4 |
| Popis: | Cellular and intestinal absorption of naked oligonucleotides (ONs) is limited and still remains a developmental challenge. A previous report in the literature suggests that ON absorption occurs via a paracellular mechanism. The aim of this study was to test this hypothesis using rat and human intestine in a Ussing chamber and in Caco-2 cells. Transport of a (35)S-labelled mixed backbone ON (MBO) across human or rat intestinal tissue or across Caco-2 cells was measured after a 2-h incubation in the presence or absence of increasing MBO concentrations or with uptake inhibitors and enhancers. MBO intestinal absorption was compared with an internal standard, mannitol. (35)S-MBO demonstrated very little absorption ( |
| Databáze: | OpenAIRE |
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