Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA
| Autor: | Jack D. Keene, Ronald C. Herman, Manfred Schubert, Robert A. Lazzarini |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Base Sequence
Genes Viral Polyadenylation Immunology Nucleic acid sequence RNA Biology biology.organism_classification Microbiology Molecular biology Vesicular stomatitis Indiana virus Vesicular Stomatitis RNA silencing Defective interfering particle Vesicular stomatitis virus Virology Insect Science Nucleic Acid Conformation RNA Viral RNA Messenger Poly A Gene Research Article |
| Zdroj: | Journal of Virology. 34:550-559 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.34.2.550-559.1980 |
| Popis: | The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase were identified and isolated by specific duplex formation between 5' terminally labeled oligonucleotides from a small single-stranded defective interfering particle RNA and L gene mRNA. The L gene ends at position 60 from the 5' terminus of the vesicular stomatitis genome. The data demonstrated that the first seven adenine residues in the polyadenylic acid tail of L gene mRNA may be coded for in the genome and suggested that the viral transcriptase itself may carry out polyadenylation, possibly by chattering at the uridine-rich sequence at the end of the L gene. Analysis of the 5'-terminal sequence of vesicular stomatitis virus genomic RNA revealed that it might fold into a complex secondary structure with possibly 62% of the bases paired. |
| Databáze: | OpenAIRE |
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