Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle

Autor: Xiao-Xin Yan, Bing Ni, Ji Li, Wei Wang, Shusheng Zhang, Zuoxian Lin, Xiaobo Han, Kexin Peng, Aishi Song, Yuying Zhang, Lin-Ping Wu, Jiarong Huang, Omar Mukama, Rongqi Huang, Sihao Deng, Weiyue Deng, Jean du Dieu Habimana, Zhiyuan Li
Rok vydání: 2021
Předmět:
Zdroj: Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-11 (2021)
Stem Cell Research & Therapy
ISSN: 1757-6512
DOI: 10.1186/s13287-021-02614-0
Popis: Background Alopecia areata (AA) is a common autoimmune hair loss disease with increasing incidence. Corticosteroids are the most widely used for hair loss treatment; however, long-term usage of hormonal drugs is associated with various side effects. Mesenchymal stem cells (MSCs) therapy has been studied extensively to curb autoimmune diseases without affecting immunity against diseases. Methods Hair follicle-derived MSCs (HF-MSCs) were harvested from the waste material of hair transplants, isolated and expanded. The therapeutic effect of HF-MSCs for AA treatment was investigated in vitro AA-like hair follicle organ model and in vivo C3H/HeJ AA mice model. Results AA-like hair follicle organ in vitro model was successfully established by pre-treatment of mouse vibrissa follicles by interferon-γ (IFN-γ). The AA-like symptoms were relieved when IFN-γ induced AA in vitro model was co-cultured with HF-MSC for 2 days. In addition, when skin grafted C3H/HeJ AA mice models were injected with 106 HF-MSCs once a week for 3 weeks, the transcription profiling and immunofluorescence analysis depicted that HF-MSCs treatment significantly decreased mouse hair loss and reduced inflammation around HF both in vitro and in vivo. Conclusions This study provides a new therapeutic approach for alopecia areata based on HF-MSCs toward its future clinical application.
Databáze: OpenAIRE
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