Microbial population dynamics in a thermophilic methane digester fed with garbage
| Autor: | Jihoon Cheon, Taira Hidaka, H. Tsuno, Hiromoto Koshikawa, Feng Hong |
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| Rok vydání: | 2007 |
| Předmět: |
Operational taxonomic unit
Environmental Engineering Population Dynamics Population Biology Polymerase Chain Reaction Bacteria Anaerobic Bioreactors Species Specificity RNA Ribosomal 16S Cluster Analysis Food science education Phylogeny DNA Primers Water Science and Technology education.field_of_study Ecology Bacillus infernus Thermophile Biodiversity Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Refuse Disposal Anaerobic digestion Microbial population biology Methane Sludge |
| Zdroj: | Water Science and Technology. 55:175-182 |
| ISSN: | 1996-9732 0273-1223 |
| Popis: | The diversity of microbial communities in three full-scale thermophilic anaerobic digesters which treated garbage, sewage sludge and livestock wastes (hereafter called TGD, TSD and TLD, respectively) was investigated using 16S rDNA clone libraries in triplicate. The population dynamics of TGD were also studied. The purposes were to show the microbial diversity in each reactor and to suggest which key microbes in a thermophilic methane digester fed with garbage, including a check of reproducibility and the suggestion of an error range in this molecular biology method. 736 clones were identified, and the maximum error was estimated to be around ±10% for the same OTU (operational taxonomic unit) and for most detected OTUs. The most frequently detected OTU shows a close relationship to Uncultured bacterium clone MBA08,Unidentified bacterium clone TUG22 and Uncultured archaeal symbiont PA204 in TGD, TSD and TLD, respectively. The microbial population dynamics in TGD were studied over a period of 90 days, and the occupying ratios of Bacillus infernus and Methanothermobacter wolfeii were shown to change with the change in VFA concentration. From the dynamic change and characteristics of the microbes, it is concluded that Bacillus infernus and Methanothermobacter wolfeii played an important role and were recommended as key microbes in TGD. |
| Databáze: | OpenAIRE |
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