A cDNA clone for β-caryophyllene synthase from Artemisia annua
| Autor: | John E. Crock, Rodney Croteau, Yu Cai, Zhi-Xin Lin, Xiao-Ya Chen, Jun-Wei Jia |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Artemisia annua Plant Science Horticulture medicine.disease_cause Sesquiterpene Biochemistry chemistry.chemical_compound Gene Expression Regulation Plant Complementary DNA Gene expression medicine RNA Messenger Cloning Molecular Molecular Biology Escherichia coli Polycyclic Sesquiterpenes Molecular Structure biology ATP synthase food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Enzymes Elicitor chemistry Enzyme Induction biology.protein Heterologous expression Plant Structures Sesquiterpenes |
| Zdroj: | Phytochemistry. 61:523-529 |
| ISSN: | 0031-9422 |
| Popis: | An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to beta-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, beta-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The beta-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for beta-caryophyllene in plant defense. |
| Databáze: | OpenAIRE |
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