Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor α Expression in Osteosarcoma Cell Lines
| Autor: | Renata Veselská, Dalibor Valík, Jaroslav Sterba, Silvia Paukovcekova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
calcitriol
retinoic acid receptor α Retinoic acid Calcitriol receptor lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine Antineoplastic Combined Chemotherapy Protocols polycyclic compounds lcsh:QH301-705.5 Spectroscopy 0303 health sciences Retinoic Acid Receptor alpha General Medicine Vitamins calcidiol 3. Good health Computer Science Applications all-trans retinoic acid 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) medicine.drug Calcitriol Antineoplastic Agents Tretinoin Catalysis Article Inorganic Chemistry 03 medical and health sciences Downregulation and upregulation Cell Line Tumor osteosarcoma medicine Humans vitamin D receptor Physical and Theoretical Chemistry Molecular Biology neoplasms 030304 developmental biology Calcifediol Organic Chemistry Retinoic acid receptor Nuclear receptor chemistry lcsh:Biology (General) lcsh:QD1-999 Cell culture Retinoic acid receptor alpha Cancer research Receptors Calcitriol Drug Screening Assays Antitumor |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 6591, p 6591 (2020) International Journal of Molecular Sciences Volume 21 Issue 18 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The main objective of this study was to analyze changes in the antiproliferative effect of vitamin D3, in the form of calcitriol and calcidiol, via its combined application with all-trans retinoic acid (ATRA) in osteosarcoma cell lines. The response to treatment with calcitriol and calcidiol alone was specific for each cell line. Nevertheless, we observed an enhanced effect of combined treatment with ATRA and calcitriol in the majority of the cell lines. Although the levels of respective nuclear receptors did not correlate with the sensitivity of cells to these drugs, vitamin D receptor (VDR) upregulation induced by ATRA was found in cell lines that were the most sensitive to the combined treatment. In addition, all these cell lines showed high endogenous levels of retinoic acid receptor &alpha (RAR&alpha ). Our study confirmed that the combination of calcitriol and ATRA can achieve enhanced antiproliferative effects in human osteosarcoma cell lines in vitro. Moreover, we provide the first evidence that ATRA is able to upregulate VDR expression in human osteosarcoma cells. According to our results, the endogenous levels of RAR&alpha and VDR could be used as a predictor of possible synergy between ATRA and calcitriol in osteosarcoma cells. |
| Databáze: | OpenAIRE |
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