Use of activity monitors for assessment of pruritus in an acute model of canine atopic dermatitis
| Autor: | Jean Steffan, Christine Prost, Chiara Nenci, Wolfgang Seewald, Rachel Schwab-Richards, Petra Roosje |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Video Recording Positive control medicine.disease_cause Gastroenterology Dermatitis Atopic Allergen challenge Allergen Dogs Internal medicine medicine Animals Antigens Dermatophagoides Dog Diseases Skin Cross-Over Studies General Veterinary Behavior Animal Abdominal skin business.industry Parallel study Atopic dermatitis Allergens medicine.disease Crossover study Surgery Disease Models Animal Prednisolone Female business medicine.drug |
| Zdroj: | Veterinary dermatology. 25(5) |
| ISSN: | 1365-3164 |
| Popis: | BACKGROUND: We developed a canine model of acute atopic dermatitis to evaluate the potential of compounds to treat pruritus and skin lesions induced in Dermatophagoides farinae (Df)-sensitized dogs. HYPOTHESIS/OBJECTIVES: The aim was to investigate the effectiveness of long-term recording activity monitors to assess pruritus induced by allergen challenges. ANIMALS: Thirty-two Df-sensitized laboratory dogs. METHODS: In two blinded crossover studies, 28 Df-sensitized dogs were challenged on 3 days with a Df slurry applied to clipped abdominal skin. Dogs were treated with a positive control (prednisolone 1 mg/kg once daily for 5 days, starting 1 day before challenge) or left untreated; all were fitted with activity monitors. To confirm pruritus, a parallel study with four dogs was conducted, filming the dogs before and during challenge and assessing the film for pruritic behaviour. RESULTS: The activity of dogs treated with prednisolone was significantly lower between 00.00 and 03.00 h and between 03.00 and 06.00 h compared with untreated dogs (repeated-measures ANCOVA; P < 0.0001). To determine whether the recorded night-time activity corresponded to pruritic manifestations, we compared activity monitor and video recordings of four dogs for two periods (16.30-20.30 and 24.00-03.00 h) before and during a Df challenge. The correlation between night-time activity monitor activity and observed pruritic behaviour was highly significant (test of correlation coefficient versus zero: r = 0.57, P < 0.0001). CONCLUSIONS AND CLINICAL IMPORTANCE: Determination of night-time activity with activity monitors after allergen challenge appears to be an objective and practical way to assess pruritus in this experimental model of canine atopic dermatitis. |
| Databáze: | OpenAIRE |
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