Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa

Autor: N. Tekin Onder, M. Berk Toker, Selim Alcay, Elif Gokce
Přispěvatelé: Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı., Alçay, Selim, Toker, M. Berk, Önder, N. Tekin, Gökçe, Elif, ABA-6294-2020
Rok vydání: 2017
Předmět:
Male
Physiology
Royal jelly
Freeze thawing
DNA fragmentation
Antioxidants
Cryopreservation
law.invention
Plasma
Cryoprotective Agents
0302 clinical medicine
law
Lecithins
Cryoprotective agent
Semen frozen
LIncubation resilience
Incubation
Insemination
Artificial
Sperm motility
Goat semen
030219 obstetrics & reproductive medicine
Spermatozoon
Spermatozoon motility
Goats
Fatty Acids
Extender
04 agricultural and veterinary sciences
General Medicine
Semen
Semen Extenders
Veterinary
medicine.anatomical_structure
Goat
Soybean Proteins
Sperm Motility
Ram sperm parameters
Soybean protein
General Agricultural and Biological Sciences
Acrosome
food.ingredient
education
Artificial insemination
Biology
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
food
Animal science
Sperm preservation
In Situ Nick-End Labeling
medicine
Animals
Yolk-based extender
Life sciences & biomedicine - other topics
Drug effects
Animal
Cell Membrane
0402 animal and dairy science
DNA
TUNEL assay
Fatty acid
Nonhuman
040201 dairy & animal science
Sperm
Phosphatidylcholine
Fertility
Concentration (parameters)
Immunology
Semen analysis
Ffreeze thawing
Egg-yolk
Controlled study
DNA Damage
Semen Preservation
Zdroj: Cryobiology. 74:81-85
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2016.11.011
Popis: The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.
Databáze: OpenAIRE
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