| Autor: |
Jianmei Yang, Xiaoyan Gan, Xinmei Song, Ruo Yuan, Yun Xiang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 1233:340515 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/j.aca.2022.340515 |
| Popis: |
Sensitive and selective detection of neutrophil gelatinase-associated lipocalin (NGAL) is critical for the prediction and early diagnosis of acute renal injury. In this work, the establishment of an aptamer-based, highly sensitive and label-free method for detecting NGAL in diluted human serums via metal ion-dependent DNAzyme- and exonuclease III (Exo III)-triggered recycling signal amplification cascades is described. NGAL binds with the aptamer strands in the DNAzyme/aptamer duplexes and results in the liberation of the metal ion-dependent DNAzyme sequences to cleave the hairpin signal probes on the electrode to liberate the G-quadruplex and intermediate strands. The released intermediate strands further complement with the DNAzyme/aptamer duplexes to form favorable substrate for Exo III, which digests the duplexes to release the DNAzyme strands to initiate the cascaded recycling cycles for the yield of plenty of G-quadruplex strands. Hemin can associate with G-quadruplex strands to produce many G-quadruplex/hemin complexes and electrochemical reduction of hemin thus generates highly amplified current for detecting NGAL with the detection limit of 4.45 ng mL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect