TGF-β1 and fibroblast growth factor-1 modify fibroblast growth factor-2 production in type II cells
| Autor: | Philip L. Sannes, Jody Khosla, Cheng-Ming Li, Paul Hoyle, Ines Pagan |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Physiology Pulmonary Fibrosis medicine.medical_treatment Basic fibroblast growth factor Gene Expression Biology Fibroblast growth factor chemistry.chemical_compound Biosynthesis Transforming Growth Factor beta Fibrosis Physiology (medical) Internal medicine Pulmonary fibrosis medicine Animals RNA Messenger Receptor Fibroblast Growth Factor Type 1 Cells Cultured Dose-Response Relationship Drug DNA synthesis Receptor Protein-Tyrosine Kinases Cell Biology medicine.disease Receptors Fibroblast Growth Factor Rats Inbred F344 Rats Specific Pathogen-Free Organisms Cell biology Pulmonary Alveoli Cytokine Endocrinology chemistry Fibroblast Growth Factor 1 Fibroblast Growth Factor 2 Transforming growth factor |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 279:L1038-L1046 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.2000.279.6.l1038 |
| Popis: | Fibroblast growth factor (FGF)-2, which stimulates DNA synthesis by type II cells in the lung, has been shown to be regulated by transforming growth factor (TGF)-β1, an important inflammatory cytokine, in vascular epithelium. The goal of this study was to determine if FGF-2 production by alveolar type II cells is modulated by TGF-β1 or FGF-1, which also stimulates DNA synthesis by type II cells. Isolated rat type II cells were exposed to 0–40 ng/ml of TGF-β1 or 0–500 ng/ml of FGF-1 in serum-free medium for 1–5 days. With a specific immunoassay, significant increases of FGF-2 protein in type II cell lysates to levels above those in control cells were achieved after 1 day of exposure to 100 ng/ml of FGF-1 and after 3 days of treatment with 8 ng/ml of TGF-β1. Similarly, transcripts for FGF-2 were dramatically increased above those in control cells with TGF-β1 or FGF-1, as were those for FGF receptor-1. These results demonstrate important regulatory links between FGF-2 and both TGF-β1 and FGF-1 in the alveolar epithelium that could contribute to the regulation of normal cell turnover, development, and the repair processes after injury in the lung. |
| Databáze: | OpenAIRE |
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