Modulation of Rat Liver Regeneration after Partial Hepatectomy by Dietary Cholesterol
| Autor: | Petra Mocková, Jolana Cermanova, Helena Živná, Vladimir Palicka, Lenka Žaloudková, Stanislav Micuda, Pavel Živný |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty 050402 sociology lcsh:Medicine Absorption (skin) Cholesterol Dietary 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ACAT-2 0504 sociology Internal medicine medicine Animals Hepatectomy rat Carbon Radioisotopes Rats Wistar Triglycerides 030203 arthritis & rheumatology Triglyceride DNA synthesis Chemistry Cholesterol Regeneration (biology) 05 social sciences lcsh:R cholesterol LDL receptor General Medicine DNA Liver regeneration partial hepatectomy Liver Regeneration Rats Lipoproteins LDL Endocrinology Liver lipids (amino acids peptides and proteins) Lipoprotein Sterol O-Acyltransferase |
| Zdroj: | Acta Medica, Vol 61, Iss 1, Pp 22-28 (2018) |
| ISSN: | 1211-4286 |
| Popis: | Introduction: The aim of study was to evaluate impact of long-term dietary cholesterol overload on the cholesterol homeostasis and liver regeneration. Material and Methods: Serum lipid parameters,14C-cholesterol incorporation, liver DNA synthesis and protein expression was determined in partially hepatectomized (PH) rats fed with a standard (SLD) or hypercholesterolemic (CHOL) diet. Results: 29-day intake of CHOL diet before PH produced increase in serum total cholesterol, LDL lipoprotein, and triglyceride concentration. PH provoked decrease in serum total cholesterol and triglyceride concentration in both groups. PH was associated with increase in serum ALT activity more pronounced in CHOL animals. Hepatic DNA synthesis was increased after PH in both groups, but lower in CHOL. Hypercholesterolemic diet reduced the absorption of radiolabelled cholesterol in intestine and then activity in blood and liver. The14C-cholesterol hepatic activities tend to increase after PH in both groups. CHOL diet produced up-regulation of Acyl-CoA:cholesterol acyltransferase-2 protein expression. PH was associated with increase of LDL receptor and Acyl-CoA:cholesterol acyltransferase-2 protein expression in both dietary groups. Discussion: Liver regeneration after PH is negatively influenced by CHOL diet. The increased uptake of cholesterol in the liver after PH associated with up-regulation of LDL receptor protein expression suggests preferential use of extrahepatic cholesterol by the liver. |
| Databáze: | OpenAIRE |
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