Metabolic and transcriptional transitions in barley glumes reveal a role as transitory resource buffers during endosperm filling
| Autor: | Julien Hollmann, Hans Weber, Winfriede Weschke, Stefan Kohl, Alexander Erban, David Riewe, Joachim Kopka |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Transcription
Genetic Physiology Plant Science barley (Hordeum vulgare L. cv. Barke) Endosperm chemistry.chemical_compound nitrogen (N) remobilization Gene Expression Regulation Plant Botany WRKY transcription factors NAC transcription factors Abscisic acid Vascular tissue Plant Proteins biology Jasmonic acid Gene Expression Profiling fungi Glume jasmonic acid food and beverages Hordeum biology.organism_classification WRKY protein domain chemistry ABA glumes Hordeum vulgare sense organs N transport seed development Research Paper Abscisic Acid Transcription Factors |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Highlight The development and metabolism of barley glumes is tightly associated with grain filling and filial sink strength, which coordinate developmental phase changes in the glumes via metabolic, hormonal, and transcriptional control. During grain filling in barley (Hordeum vulgare L. cv. Barke) reserves are remobilized from vegetative organs. Glumes represent the vegetative tissues closest to grains, senesce late, and are involved in the conversion of assimilates. To analyse glume development and metabolism related to grain filling, parallel transcript and metabolite profiling in glumes and endosperm were performed, showing that glume metabolism and development adjusts to changing grain demands, reflected by specific signatures of metabolite and transcript abundances. Before high endosperm sink strength is established by storage product accumulation, glumes form early, intermediary sink organs, shifting then to remobilizing and exporting source organs. Metabolic and transcriptional transitions occur at two phases: first, at the onset of endosperm filling, as a consequence of endosperm sink activity and assimilate depletion in endosperm and vascular tissues; second, at late grain filling, by developmental ageing and senescence. Regulation of and transition between phases are probably governed by specific NAC and WRKY transcription factors, and both abscisic and jasmonic acid, and are accompanied by changed expression of specific nitrogen transporters. Expression and metabolite profiling suggest glume-specific mechanisms of assimilate conversion and translocation. In summary, grain filling and endosperm sink strength coordinate phase changes in glumes via metabolic, hormonal, and transcriptional control. This study provides a comprehensive view of barley glume development and metabolism, and identifies candidate genes and associated pathways, potentially important for breeding improved grain traits. |
| Databáze: | OpenAIRE |
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