Isolation and characterization of the gene encoding the chloroplast-type ferredoxin component of carbazole 1,9a-dioxygenase from a putative Kordiimonas sp
| Autor: | Yoshihiko Ito, Rintaro Maeda, Takanori Ishii, Toshio Omori, Azham Zulkharnain, Kenichi Iwata |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Chloroplasts Sequence analysis Molecular Sequence Data Carbazoles Gene Expression Bioengineering Biology medicine.disease_cause Applied Microbiology and Biotechnology Dioxygenases Bacterial Proteins Dioxygenase Gene Order Gene cluster Escherichia coli medicine Consensus sequence Cloning Molecular Peptide sequence Ferredoxin Alphaproteobacteria Sequence Homology Amino Acid Sequence Analysis DNA General Medicine biology.organism_classification Recombinant Proteins Kordiimonas gwangyangensis Biochemistry Multigene Family Ferredoxins Biotechnology |
| Zdroj: | Biotechnology Letters. 32:1725-1731 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-010-0358-x |
| Popis: | Carbazole (CAR)-degrading genes (carRAaCBaBb) were isolated from marine CAR-degrading isolate strain OC9 (probably Kordiimonas gwangyangensis) using shotgun cloning experiments and showed 35-65% similarity with previously reported CAR-degrading genes. In addition, a ferredoxin-like gene (carAc) was found downstream of carR, although it was not homologous with any reported ferredoxin components of the CAR 1,9a-dioxygenase (CARDO) system. The carAc-deduced amino acid sequence possessed consensus sequences for chloroplast-type iron-sulfur proteins for binding the [2Fe-2S] cluster. These car genes were arranged in the order of carAcRAaCBaBb, but carRAc and carAaCBaBb genes were the opposite orientation. Escherichia coli JM109 cells harboring pBOC91 (carAa) converted CAR to 2'-aminobiphenyl-2,3-diol at a ratio of 12%, and the transformation ratio of CAR increased from 12 to 100% when carAc was added, indicating that CarAc is the ferredoxin component of the CARDO system in strain OC9. This is the first finding of a chloroplast-type ferredoxin component in a CARDO system. Biotransformation tests with aromatic compounds revealed that the strain OC9 CarAaAc showed activity with polycyclic aromatic hydrocarbons and dioxin compounds and exhibited significant activity for fluorene, unlike previously reported CARDOs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink