Culturing periprosthetic tissue in blood culture bottles results in isolation of additional microorganisms
| Autor: | Anton Buiting, Wouter van den Bijllaardt, Marco Janssens, Marc Peijs, Anne Q Reuwer, Olav P van der Jagt |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Microbiological Techniques Reoperation medicine.medical_specialty food.ingredient Prosthesis-Related Infections Time Factors Microorganism Joint Prosthesis 030106 microbiology Periprosthetic Sensitivity and Specificity Microbiology Arthroplasty 03 medical and health sciences 0302 clinical medicine food Medical microbiology Culture Techniques medicine Agar Humans Blood culture 030212 general & internal medicine Prospective Studies Prospective cohort study Aged Aged 80 and over medicine.diagnostic_test Bacteria business.industry General Medicine Gold standard (test) Isolation (microbiology) Infectious Diseases business |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 38(2) |
| ISSN: | 1435-4373 |
| Popis: | Despite low sensitivity, culture of periprosthetic tissue (PPT) specimens on agars and in broths has traditionally been used for the detection of causative microorganisms in patients suspected for prosthetic joint infection (PJI). The aim of this study was to evaluate the added diagnostic value of culturing PPT in blood culture bottles (BCB) over the conventional combination of standard agar and broth alone. This prospective cohort study was conducted over a 12-month period and included consecutive patients undergoing revision arthroplasty. Overall, 113 episodes from 90 subjects were studied; 45 subjects (50.0%) met the Infectious Diseases Society of America (IDSA) criteria for PJI, of whom the majority (75.6%) had an acute infection. Sensitivity and specificity of culture were assessed using IDSA criteria for PJI as gold standard. Although the increase in sensitivity from 84.44 (CI 70.54; 93.51) to 93.33% (81.73; 98.60) was not significant, added diagnostic value of culturing PPT in BCBs was demonstrated by the significantly higher number of detected pathogens in culture sets with BCBs compared to culture without BCBs (61 pathogens in conventional set versus 89 when BCBs were included for 57 PJI episodes, P = |
| Databáze: | OpenAIRE |
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