A novel fungal beta-propeller phytase from nematophagous Arthrobotrys oligospora: characterization and potential application in phosphorus and mineral release for feed processing
| Autor: | Yongzhong Wang, Kangliang Sheng, Zhen Shen, Xianjuan Hou, Jingmin Wang, Xiaowei Kong, Na Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Animal feed
Soybean meal Wheat flour lcsh:QR1-502 chemistry.chemical_element Bioengineering Phosphorus and mineral release Feed processing Nematophagous fungi Applied Microbiology and Biotechnology lcsh:Microbiology Nematophagous fungus Pichia pastoris 03 medical and health sciences Arthrobotrys oligospora Animals Humans Food science 030304 developmental biology 0303 health sciences 6-Phytase biology 030306 microbiology Phosphorus Research Fungi biology.organism_classification Phytase Enzyme assay chemistry biology.protein Food Microbiology Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 19, Iss 1, Pp 1-13 (2020) |
| ISSN: | 1475-2859 |
| Popis: | Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fungal-derived BPP phytase. We identified a phytase highly differentially expressed only in the parasitic stage of a nematophagous fungus, Arhtrobotrys oliogospora, during the development of the 3D traps. We found that this phytase was homologous to the known bacterial BPP phytase, thus we referred the new phytase to Aophytase. The heterologous expression of codon-optimized Aophytase gene in Pichia pastoris was successfully investigated to yield recombinant Aophytase (r-Aophytase) with high specific enzyme activity of 74.71 U/mg, much higher than those of recombinant BPP phytases derived bacteria. The kinetic parameters of the r-Aophytase, the optimum pH and temperature, as well as the effects of surfactant, EDTA and different ions on its enzyme activity were further investigated. The potential utilization of r-Aophytase in feed processing was finally explored. We found that the optimal pH value was about 7.5, and the optimal temperature was 50 °C.; r-Aophytase significantly increased the release of inorganic phosphorus from soybean meal, and improved the release of soluble minerals from the durum wheat flour and finger millet flour. The findings indicate its potential utilization in the feed processing to ameliorate nutritional value of cereals and animal feed in the future. |
| Databáze: | OpenAIRE |
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