Non-muscle myosin as target antigen for human autoantibodies in patients with hepatitis C virus-associated chronic liver diseases
| Autor: | H. Imai, Eng M. Tan, C. A. Von Muhlen, Edward K. L. Chan, Kendo Kiyosawa, Carol L. Peebles |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Hepatitis C virus Blotting Western Immunology Myosins Microfilament Chronic liver disease Immunofluorescence medicine.disease_cause Autoantigens Peptide Mapping Antigen Myosin medicine Humans Immunology and Allergy Aged Autoantibodies biology medicine.diagnostic_test Autoantibody Middle Aged medicine.disease Hepatitis C Virology Chronic Disease biology.protein Female Antibody Research Article |
| Zdroj: | Clinical and Experimental Immunology. 100:67-74 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.1995.tb03605.x |
| Popis: | SUMMARYThree patients with hepatitis C virus (HCV)-related chronic liver disease were shown to have autoantibodies strongly reacting with cytoskeletal fibres of non-muscle cells. The heavy chain of non-muscle myosin microfilament was the main target for those autoantibodies, as determined by (i) cell and tissue immunofluorescence studies showing colocalization with an anti-myosin antibody prototype; (ii) primary reactivity in immunoblotting with a 200-kD protein, using either MOLT-4 cells, human platelets, or affinity-purified non-muscle myosin as antigen extract; and (iii) immunoblotting of similar immunoreactive fragments in papain-digested MOLT-4 cell extracts, by using those human sera and antibody prototype. Autoantibodies to non-muscle myosin heavy chain were not previously reported in patients with chronic liver diseases, especially in those associated with HCV infection. |
| Databáze: | OpenAIRE |
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