Curing Action of Sodium Dodecyl Sulfate on a Proteus mirabilis R + Strain
| Autor: | Manabu Inuzuka, Munemitsu Tomoeda, Shizuko Anto, Mariko Konishi |
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| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Lysis Extrachromosomal Inheritance Genetics and Molecular Biology Biology medicine.disease_cause Microbiology chemistry.chemical_compound Sulfanilamides Escherichia coli medicine Sodium dodecyl sulfate Proteus mirabilis Molecular Biology Nitrosoguanidines Dihydrostreptomycin Sulfate Strain (chemistry) Transfer factor Chloramphenicol Genetic Variation Sodium Dodecyl Sulfate Drug Resistance Microbial Tetracycline biology.organism_classification chemistry Biochemistry Conjugation Genetic Acridines Mutagens medicine.drug |
| Zdroj: | Journal of Bacteriology. 120:1158-1163 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.120.3.1158-1163.1974 |
| Popis: | Growth of Proteus mirabilis harboring R100-1 ( fi + drd str r cml r tet r sul r ) factors in Penassay broth containing sodium dodecyl sulfate (SDS) leads to the loss of all or part of the genetic elements in high frequencies. In media containing SDS at concentrations as low as 0.03%, both lysis of R + cells and elimination of the R factors occur at high frequencies. Appearance of drug-susceptible cells in R + cultures occurs during the exponential phase of growth; however, the frequencies of susceptible cells increase substantially after the culture reaches the stationary phase. Reconstruction experiments, coupled with other observations, suggest that the major factor in altering the frequency of drug-susceptible variants is the greater resistance of the variants to the lytic action of SDS. This resistance correlates in most cases with the loss of the transfer functions in the resistance transfer factor. |
| Databáze: | OpenAIRE |
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