Primary human hepatocytes versus hepatic cell line: assessing their suitability for in vitro nanotoxicology
| Autor: | Vicki Stone, Michael B. Ward, Birgit K Gaiser, Ali Kermanizadeh |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Cell Survival Surface Properties Primary Cell Culture Biomedical Engineering 02 engineering and technology Animal Testing Alternatives Toxicology Lethal Dose 50 03 medical and health sciences Microscopy Electron Transmission Albumins Cell Line Tumor Toxicity Tests medicine Humans Particle Size Primary cell Cytotoxicity 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug Albumin 021001 nanoscience & nanotechnology Molecular biology Endocytosis Dose–response relationship medicine.anatomical_structure Cell culture Nanotoxicology Hepatocyte Hepatocytes Microscopy Electron Scanning Hepatic stellate cell Cytokines Nanoparticles 0210 nano-technology |
| Zdroj: | Nanotoxicology |
| ISSN: | 1743-5404 1743-5390 |
| DOI: | 10.3109/17435390.2012.734341 |
| Popis: | The use of hepatocyte cell lines as a replacement for animal models have been heavily criticised mainly due to low expression of metabolism enzymes. This study compares primary human hepatocytes with the C3A cell line and with respect to their response to a panel of nanomaterials (NMs; two ZnO, two MWCNTs, one Ag and one positively functionalised TiO2). The cell line was very comparable with the primary hepatocytes with regards to their cytotoxic response to the NMs (Ag > uncoated ZnO > coated ZnO). The LC50 was not attained in the presence of the MWCNTs and the TiO2 NMs. All NMs significantly increased IL-8 production, with no change in levels of TNF-α and IL-6. Albumin production was measured as an indicator of hepatic function. The authors found no change in levels of albumin with the exception of the coated ZnO NM at the LC50 concentration. NM uptake was similar for both the primary hepatocytes and C3A cells as investigated by TEM. Meanwhile, the authors confirmed greater levels of CYP450 activity in untreated primary cells. This study demonstrates that the C3A cell line is a good model for investigating NM-induced hepatocyte responses with respect to uptake, cytotoxicity, pro-inflammatory cytokine production and albumin production. |
| Databáze: | OpenAIRE |
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