The influence of allogeneic or syngeneic cell surface backgrounds on the antibody response of mice to Rabbit Fab' fragments
| Autor: | R. Bruce Acres, Julia G. Levy |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
C57BL/6
Immunology Cell Hemolytic Plaque Technique Spleen H antigen Immunoglobulin Fab Fragments Mice Immune system Antigen Histocompatibility Antigens medicine Animals Lymphocytes Incubation Virus quantification biology biology.organism_classification Molecular biology Mice Inbred C57BL medicine.anatomical_structure Mice Inbred DBA Antibody Formation Mice Inbred CBA Female |
| Zdroj: | Cellular Immunology. 31:216-224 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(77)90023-5 |
| Popis: | Mice were immunized with antigen (Rabbit Fab' fragments) attached to syngeneic, or f 1 (semi-syngeneic) irradiated spleen cells. Specific anti-rabbit Fab' plaque forming cell numbers showed that the response towards antigen on syngeneic or F 1 cells, was significantly lower than that towards the same antigen on allogeneic cells. By subsequent in vitro incubation of immune spleen cells with antigen followed by plaque assay, it was found that those spleen cells exhibiting lowered plaque forming cell numbers initially, (i.e., those mice immunized with antigen on syngeneic or F 1 cell surfaces) showed, after incubation, a response equal to or greater than those cells which initially (before in vitro incubation) demonstrated a larger response (i.e. those mice immunized with antigen on allogeneic cells). |
| Databáze: | OpenAIRE |
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