Greb1 is required for axial elongation and segmentation in vertebrate embryos
| Autor: | Annalisa Vezzaro, David Ish-Horowicz, Richard Mitter, Ravindra Singh Prajapati |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Mesoderm
Brachyury animal structures QH301-705.5 Science Population General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine FGF8 Somitogenesis medicine Paraxial mesoderm somites Biology (General) education Zebrafish tailbud neural tube 030304 developmental biology Computational & Systems Biology progenitors Chemical Biology & High Throughput 0303 health sciences education.field_of_study biology Genome Integrity & Repair Morphant Tumour Biology biology.organism_classification Cell biology axial stem cells clock medicine.anatomical_structure embryonic structures General Agricultural and Biological Sciences Genetics & Genomics transcriptome 030217 neurology & neurosurgery Research Article |
| Zdroj: | Biology Open, Vol 9, Iss 2 (2020) Biology Open |
| ISSN: | 2046-6390 |
| Popis: | During vertebrate embryonic development, the formation of axial structures is driven by a population of stem-like cells that reside in a region of the tailbud called the chordoneural hinge (CNH). We have compared the mouse CNH transcriptome with those of surrounding tissues and shown that the CNH and tailbud mesoderm are transcriptionally similar, and distinct from the presomitic mesoderm. Amongst CNH-enriched genes are several that are required for axial elongation, including Wnt3a, Cdx2, Brachyury/T and Fgf8, and androgen/oestrogen receptor nuclear signalling components such as Greb1. We show that the pattern and duration of tailbud Greb1 expression is conserved in mouse, zebrafish and chicken embryos, and that Greb1 is required for axial elongation and somitogenesis in zebrafish embryos. The axial truncation phenotype of Greb1 morphant embryos can be explained by much reduced expression of No tail (Ntl/Brachyury), which is required for axial progenitor maintenance. Posterior segmentation defects in the morphants (including misexpression of genes such as mespb, myoD and papC) appear to result, in part, from lost expression of the segmentation clock gene, her7. Summary: The Greb1 transcriptional cofactor is expressed in the vertebrate tailbud and required for axial extension and segmentation of developing vertebrate embryos. |
| Databáze: | OpenAIRE |
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