Molecular and structural composition of phospholipid membranes in livers of marine and freshwater fish in relation to temperature
| Autor: | John E. Halver, Indranil Dey, Csaba Buda, T Wiik, Tibor Farkas |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Spectrophotometry
Infrared Membrane lipids Acclimatization Phospholipid Fluorescence Polarization Biology chemistry.chemical_compound Membrane Lipids Species Specificity Phosphatidylcholine Animals Phospholipids Phosphatidylethanolamine chemistry.chemical_classification Multidisciplinary Fourier Analysis Cell Membrane Fatty Acids Fishes Temperature Fatty acid biology.organism_classification Oleic acid chemistry Biochemistry Liver Docosahexaenoic acid Freshwater fish Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 90(16) |
| ISSN: | 0027-8424 |
| Popis: | The compositions and physical states of the liver phospholipids of marine and freshwater fish adapted to relatively constant but radically different temperatures were investigated. Fish adapted to low temperature (5-10 degrees C) accumulated more unsaturated fatty acids than those in a warm (25-27 degrees C) environment. There were no measurable differences in the gross fatty acid compositions of the total liver phospholipids from identical thermal environments. Docosahexaenoic acid (22:6) did not seem to participate in the process of adaptation. Cold adaptation was coincidental with oleic acid (18:1) accumulation, preferentially in the phosphatidylethanolamine. Determination of the molecular species composition of phosphatidylethanolamine revealed a 2- to 3-fold and 10-fold increase in the level of 18:1/22:6 and 18:1/20:5 species, respectively. ESR spectroscopy revealed a 7-10% compensation in the ordering state of native phospholipids with temperature. Combination of 16:0/22:6 phosphatidylcholine with phosphatidylethanolamines of cold-adapted marine fish showed a drastic fluidization near the C-2 segment of the bilayer, but not in the deeper regions. An appropriate combination (75:25) of phosphatidylcholines from warmth-adapted marine fish with phosphatidylethanolamines from cold-adapted marine fish mimicked a 100% adaptational efficacy in the C-2 segment as compared with the phosphatidylethanolamines of warmth-adapted marine fish. A specific role of 18:1/22:6 phosphatidylethanolamine in controlling membrane structure and physical state with thermal adaptation is proposed. |
| Databáze: | OpenAIRE |
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