Detection of Thiol Functionality and Disulfide Bond Formation by Polyoxometalate
| Autor: | Reika Aoki, Shigekazu Yano, Ikumi Nitanai, Haruto Yasumiishi, Hiroyuki Konno |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Bactenecin 010405 organic chemistry Disulfide bond Molecular Conformation General Chemistry General Medicine Glutathione Tungsten Compounds 010402 general chemistry 01 natural sciences Cyclic peptide 0104 chemical sciences chemistry.chemical_compound chemistry Intramolecular force Polymer chemistry Polyoxometalate Thiol Disulfides Sulfhydryl Compounds Deep blue |
| Zdroj: | ACS combinatorial science. 22(12) |
| ISSN: | 2156-8944 |
| Popis: | The detection of thiol functionality and intramolecular disulfide bond formation of peptides using the α-Keggin type polyoxometalate molybdenum-oxygen cluster (H3PMo12O40·nH2O) is described. Our method entails the addition of this polyoxometalate to solutions of thiol, whereupon the color of the solution changes from colorless to deep blue. Reduction of the polyoxometalate from Mo(VI) to Mo(V) occurs with concomitant oxidation of the thiol functionality, to form disulfide bonds. To exemplify the utility this phenomenon, we accomplished the oxidation of glutathione, reduced linear oxytocin, bactenecin, and α-conotoxin SI; all of which proceeded smoothly and in good conversion in 24 h to less and were accomplished by a change in the color of the reaction solutions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|