The application of next-generation sequencing to validate D12S391 microvariation

Autor: Li Jia, He Ren, Jiangwei Yan, Jin-jie Liu, Shuai Sun, Qing-xia Zhang, Yi Zhao, Ya-Cheng Liu, Chong Chen
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Forensic Science and Medicine, Vol 4, Iss 2, Pp 67-69 (2018)
ISSN: 2349-5014
Popis: Objective: In a paternity case, the D12S391 locus was reported as a mismatch. To confirm the existence of mutations and mutations come from father or mother. Methods: STR and next-generation sequencing technology were used to validate the sequence. Results: NGS showed the loss of one adenine between the 19.3 allele of the child and allele 20 of the mother. Conclusion: The NGS can be applied in the paternity to validate the mutation.
Databáze: OpenAIRE