In vivo Assessment of APPJ Discharge on the Earthworm: Coelomic TAC and MDA Levels, Cell Death and Tissue Regeneration
| Autor: | Mohammad Hossein Sinkakarimi, Neda Gholami, Abasalt Hosseinzadeh Colagar, Farshad Sohbatzadeh |
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| Rok vydání: | 2021 |
| Předmět: |
Eisenia fetida
Programmed cell death biology Cell Death Health Toxicology and Mutagenesis Regeneration (biology) General Medicine Malondialdehyde biology.organism_classification Pollution Antioxidants Lipid peroxidation Andrology chemistry.chemical_compound Atmospheric Pressure chemistry Apoptosis In vivo Catalase biology.protein Environmental Chemistry Animals Soil Pollutants Oligochaeta |
| Popis: | The cold atmospheric pressure plasma jet (APPJ) is one of the effective tools with medical applications that its positive effects have been reported by various researchers, including sterilization of liquid and solid surfaces, treatment of chronic wounds, treatment of cancer tumors, and blood clots. This study investigated the effects of APPJ discharge on the two species of Earthworms (Aporrectodea trapezoides and Eisenia fetida). The APPJ discharge for different time durations, zero as a blank, 5, 10, 20, and 30 seconds, on these two species. Then some of these effects on the worms, including regeneration ability to miss segments, Malondialdehyde (MDA), catalase activity (CAT), total antioxidant capacity (TAC), cell death, total proteins, and protein profile in the coelomic fluid were investigated. Results showed levels of TAC and CAT increased after plasma treatment. In addition, a significant change was not observed in MDA levels and cell death. In summary, it can be concluded that APPJ exposure up to 40 seconds didn’t inhibit effects on the TAC levels of the earthworms and the defense system was able to fight oxidative stress and by increasing the expression of some proteins APPJ lead to better regeneration. |
| Databáze: | OpenAIRE |
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