Monocyte-Derived Macrophages Prime Peripheral T Cells to Undergo Apoptosis by Cell-Cell Contact via ICAM -1 /LFA-1-Dependent Mechanism
| Autor: | Jun Ogata, Katsuhiro Zen, Junichi Masuda |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
T-Lymphocytes
T cell Immunology CD2 Antigens Apoptosis chemical and pharmacologic phenomena Biology Ligands Lymphocyte Activation Interleukin 21 Cell Adhesion medicine Humans Immunology and Allergy Cytotoxic T cell IL-2 receptor Antigen-presenting cell Cells Cultured Superantigens Cell adhesion molecule Macrophages ZAP70 hemic and immune systems Hematology Intercellular Adhesion Molecule-1 Natural killer T cell Molecular biology Lymphocyte Function-Associated Antigen-1 Cell biology medicine.anatomical_structure Immunologic Memory |
| Zdroj: | Immunobiology. 195:323-333 |
| ISSN: | 0171-2985 |
| Popis: | We designed the present study to clarify the mechanism of superantigen-induced apoptosis of human mature T cells and to elucidate the pivotal roles of monocyte-derived macrophages in induction of T cell apoptosis. Exposure of unfractionated human peripheral blood mononuclear cells to SEA, SEB or PHA elicited apoptosis in T cells after 5-day culture. In purified T cell preparations, SEB was unable to induce apoptosis, but was inductive when the purified T cells were cocultured with mono cyte-derived macrophages adhering to plastic culture dishes. Placing the T cells in the insert wells which physically separated them from the adhering macrophages resulted in a complete loss of SEB-induced apoptosis. The addition of blocking antibodies against LFA-1, ICAM-1 and CD2 to the cocultures significantly inhibited the SEB-induced T cell apoptosis. We concluded therefore that direct contact of macrophages with T cells is critical in SEB-induced T cell apoptosis, and that adhesion molecules such as LFA-1/ICAM-1 and CD2 may be involved in the mechanism of this effect. |
| Databáze: | OpenAIRE |
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