Positive Selection of a Qa-1-Restricted T Cell Receptor with Specificity for Insulin
| Autor: | Piotr Kraj, Barbara A. Sullivan, Peter E. Jensen, Leszek Ignatowicz, Dominique A. Weber |
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| Rok vydání: | 2002 |
| Předmět: |
Immunology
Receptors Antigen T-Cell Mice Transgenic Thymus Gland Streptamer Biology Lymphocyte Activation Mice Interleukin 21 Animals Insulin Immunology and Allergy Cytotoxic T cell IL-2 receptor Antigen-presenting cell Cells Cultured Interleukin 3 Antigen Presentation Hybridomas ZAP70 Histocompatibility Antigens Class I Epithelial Cells Hematopoietic Stem Cells Natural killer T cell Molecular biology Mice Inbred C57BL Phenotype Infectious Diseases T-Lymphocytes Cytotoxic |
| Zdroj: | Immunity. 17:95-105 |
| ISSN: | 1074-7613 |
| Popis: | The phenotype and development of T cells from transgenic mice expressing a T cell receptor with specificity for insulin presented by the MHC class Ib molecule Qa-1 b was investigated. Peripheral T cells from the transgenic mice express CD8 and, after activation, kill Qa-1 b -positive lymphoid target cells in the presence of soluble insulin. Thymic selection requires expression of Qa-1 b but not the dominant Qa-1-associated peptide, Qdm. In contrast to conventional T cells, selection is at least as efficient when the selecting ligand is expressed only on hematopoietic lineage cells as compared to expression on epithelial cells in the thymus. Our findings suggest that there is a dedicated population of Qa-1-restricted T cells that are selected by interaction with Qa-1 and that the cellular requirements for selection may differ from conventional T cells. |
| Databáze: | OpenAIRE |
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