High‐pressure freezing followed by freeze substitution of a complex and variable density miniorgan: the wool follicle

Autor:	Sharon Lequeux, Duane P. Harland, Mihnea Bostina, Allan Mitchell, Marina Richena, Sailakshmi Velamoor
Rok vydání:	2020
Předmět:	Histology Freeze Substitution 02 engineering and technology Pathology and Forensic Medicine Cryofixation 03 medical and health sciences Follicle Microscopy Electron Transmission Freezing Animals Vitrification 030304 developmental biology Cryopreservation 0303 health sciences Sheep Tissue Preservation Chemistry Wool 021001 nanoscience & nanotechnology Freeze substitution Transmission electron microscopy Ultrastructure Biophysics 0210 nano-technology Hair Follicle
Zdroj:	Journal of Microscopy. 278:18-28
ISSN:	1365-2818 0022-2720
Popis:	Cryofixation by high-pressure freezing (HPF) followed by freeze substitution (FS) is a preferred method to prepare biological specimens for ultrastructural studies. It has been shown to achieve uniform vitrification and ultrastructure preservation of complex structures in different cell types. One limitation of HPF is the small sample volume of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27b66ad6d4f12b71801e2418ec8f53d5 https://doi.org/10.1111/jmi.12875 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.