Trypanosoma cruzi: Characterization of in vitro and in vivo synthesized polypeptides from epimastigote forms of different strains
| Autor: | Leda Maria Cummings, Paulo Cesar Cotrim, JoséFranco da Silveira |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Reticulocytes
Immunoprecipitation Trypanosoma cruzi Immunology Antigens Protozoan Reticulocyte In vivo medicine Animals RNA Messenger Polyacrylamide gel electrophoresis Antiserum Gel electrophoresis biology General Medicine biology.organism_classification Molecular biology In vitro Molecular Weight Infectious Diseases medicine.anatomical_structure Biochemistry Protein Biosynthesis Electrophoresis Polyacrylamide Gel Parasitology Rabbits |
| Zdroj: | Experimental Parasitology. 64:354-360 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(87)90047-6 |
| Popis: | Intact RNAs were isolated from epimastigote forms of different Trypanosoma cruzi strains. Translation of the mRNAs using rabbit reticulocyte lysate and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed protein profiles comparable to those observed by labeling cells in vivo. No major interstrain differences were observed in the patterns of the polypeptides synthesized in vitro and in vivo, indicating that metabolic proteins are similar among distinct strains. Several T. cruzi polypeptides produced in the rabbit reticulocyte lysate system were immunoprecipitated by specific antisera. The patterns of antigenic polypeptides recognized by antisera raised against epimastigotes from different strains were also very similar. |
| Databáze: | OpenAIRE |
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