A PAR-1–dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte
| Autor: | Weiping Lu, C. Fiona Cullen, Russell S. Hamilton, Graeme Ball, Richard M. Parton, Hiroyuki Ohkura, Ilan Davis, Lei Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Polarity (physics)
Protein Serine-Threonine Kinases Microtubules Article Glycogen Synthase Kinase 3 03 medical and health sciences 0302 clinical medicine Microtubule Cell polarity Molecular motor medicine Animals Drosophila Proteins Research Articles 030304 developmental biology 0303 health sciences biology Biological Transport Cell Biology biology.organism_classification Oocyte Cell biology Drosophila melanogaster medicine.anatomical_structure Oocytes Nucleus 030217 neurology & neurosurgery Drosophila Protein |
| Zdroj: | The Journal of Cell Biology Parton, R, Hamilton, R, Ball, G, Yang, L, Cullen, F, Lu, W, Ohkura, H & Davis, I 2011, ' A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte ', Journal of Cell Biology, vol. 194, no. 1, pp. 121-135 . https://doi.org/10.1083/jcb.201103160 |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201103160 |
| Popis: | A PAR-1–mediated bias in microtubule organization in the Drosophila oocyte underlies posterior-directed mRNA transport. Cytoskeletal organization is central to establishing cell polarity in various cellular contexts, including during messenger ribonucleic acid sorting in Drosophila melanogaster oocytes by microtubule (MT)-dependent molecular motors. However, MT organization and dynamics remain controversial in the oocyte. In this paper, we use rapid multichannel live-cell imaging with novel image analysis, tracking, and visualization tools to characterize MT polarity and dynamics while imaging posterior cargo transport. We found that all MTs in the oocyte were highly dynamic and were organized with a biased random polarity that increased toward the posterior. This organization originated through MT nucleation at the oocyte nucleus and cortex, except at the posterior end of the oocyte, where PAR-1 suppressed nucleation. Our findings explain the biased random posterior cargo movements in the oocyte that establish the germline and posterior. |
| Databáze: | OpenAIRE |
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