Development of an Electrochemical Aptasensor for the Detection of Human Osteopontin
| Autor: | Luís G. Dias, António M. Peres, Lígia R. Rodrigues, Sofia G. Meirinho |
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| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2014 |
| Předmět: |
Science & Technology
Materials science Protein biomarkers biology Aptamer 02 engineering and technology General Medicine 021001 nanoscience & nanotechnology Electrochemistry Molecular biology Biomarker (cell) 03 medical and health sciences Microelectrode 0302 clinical medicine Thrombin stomatognathic system 030220 oncology & carcinogenesis medicine biology.protein Osteopontin Cyclic voltammetry 0210 nano-technology Engineering(all) medicine.drug |
| Zdroj: | Procedia Engineering. 87:316-319 |
| ISSN: | 1877-7058 |
| DOI: | 10.1016/j.proeng.2014.11.671 |
| Popis: | Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An RNA aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human OPN detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection li mit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin. © 2014 The Authors. Published by Elsevier Ltd. Peer-review under responsibility of the scientific committee of Eurosensors 2014. |
| Databáze: | OpenAIRE |
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